Keywords:phosphate starvation maize inhibits lateral root formation and alters gene expression the lateral root primordium zone
Authors: Li Z,Xu C,Li K,Yan S,Qu X,Zhang J,
Phosphorus (P) is an essential macronutrient for all living organisms. maize (Zea mays) is an important human food, animal feed and energy crop throughout the world, and enormous quantities of Phosphate fertilizer are required for maize cultivation. Thus, it is important to improve the efficiency of the use of phosphate fertilizer for maize.
In this study, we analyzed the maize root response to phosphate starvation and performed a transcriptomic analysis of the 1.0-1.5 cm lateral root primordium zone. In the growth of plants, the root-to-shoot ratio (R/L) was reduced in both low-phosphate (LP) and sufficient-phosphate (SP) solutions, but the ratio (R/L) exhibited by the plants in the LP solution was higher than that of the SP plants. The growth of primary roots was slightly promoted after 6 days of phosphate starvation, whereas the numbers of lateral roots and lateral root primordia were significantly reduced, and these differences were increased when associated with the stress caused by phosphate starvation. Among the results of a transcriptomic analysis of the maize lateral root primordium zone, there were two highlights: 1) auxin signaling participated in the response and the modification of root morphology under low-phosphate conditions, which may occur via local concentration changes due to the biosynthesis and transport of auxin, and LOB domain proteins may be an intermediary between auxin signaling and root morphology; and 2) the observed retardation of lateral root development was the result of co-regulation of DNA replication, transcription, protein synthesis and degradation and cell growth.
These results indicated that maize roots show a different growth pattern than Arabidopsis under low-phosphate conditions, as the latter species has been observed to halt primary root growth when the root tip comes into contact with low-phosphate media. Moreover, our findings enrich our understanding of plant responses to phosphate deficits and of root morphogenesis in maize.Related ArticlesMolecular interactions of ROOTLESS CONCERNING CROWN AND SEMINAL ROOTS, a LOB domain protein regulating shoot-borne root initiation in maize (Zea mays L.).
Philos Trans R Soc Lond B Biol Sci. 2012
Molecular interactions of ROOTLESS CONCERNING CROWN AND SEMINAL ROOTS, a LOB domain protein regulating shoot-borne root initiation in maize (Zea mays L.).
Majer C, Xu C, Berendzen KW, Hochholdinger F. Philos Trans R Soc Lond B Biol Sci. 2012 Jun 5; 367(1595):1542-51. SIZ1 regulation of phosphate starvation-induced root architecture remodeling involves the control of auxin accumulation.
Plant Physiol. 2011
SIZ1 regulation of phosphate starvation-induced root architecture remodeling involves the control of auxin accumulation.
Miura K, Lee J, Gong Q, Ma S, Jin JB, Yoo CY, Miura T, Sato A, Bohnert HJ, Hasegawa PM. Plant Physiol. 2011 Feb; 155(2):1000-12. Epub 2010 Dec 14.A role for auxin redistribution in the responses of the root system architecture to phosphate starvation in Arabidopsis.
Plant Physiol. 2005
A role for auxin redistribution in the responses of the root system architecture to phosphate starvation in Arabidopsis.
Nacry P, Canivenc G, Muller B, Azmi A, Van Onckelen H, Rossignol M, Doumas P. Plant Physiol. 2005 Aug; 138(4):2061-74. Epub 2005 Jul 22.Review Regulation of shoot and root development through mutual signaling.
Mol Plant. 2012Review
Regulation of shoot and root development through mutual signaling.
Puig J, Pauluzzi G, Guiderdoni E, Gantet P. Mol Plant. 2012 Sep; 5(5):974-83. Epub 2012 May 24.Review Transcriptional regulation of phosphate acquisition by higher plants.
Cell Mol Life Sci. 2012Review
Transcriptional regulation of phosphate acquisition by higher plants.
Jain A, Nagarajan VK, Raghothama KG. Cell Mol Life Sci. 2012 Oct; 69(19):3207-24. Epub 2012 Aug 17.